Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, cell differentiation, cell movement and cell death. MAPK signaling cascades are organized hierarchically into three-tiered modules. MAPKs are phosphorylated and activated by MAPK kinases (MAPKKs), which in turn are phosphorylated and activated by MAPKK-kinases (MAPKKKs). The MAPKKKs are in turn activated by interaction with a family of small GTPases and/or other protein kinases connecting the MAPK module to the cell surface receptor or external stimuli. The Phospho-Erk1/2 Pathway Sampler BioAssay™ Kit provides a fast and economical means to evaluate the activation status of the entire Erk1/2 pathway. The kit includes enough primary and secondary antibodies to perform two Western mini-blot experiments, as well as a specific inhibitor of MEK1/2 (U0126).
仕様
Size：5x20ul
Purity：Purified by protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Each antibody in the Phospho-Erk1/2 Pathway Sampler Kit recognizes only the phosphorylated form of its specific target.
