85-1405-91　［受注停止］Agarose DNA Fragments 50-1000bp 250g　CAS No:9012-36-6　A1005

特徴

• Agarose for DNA Fragments 50-1000bp is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels.
• Manufactured specifically for primer size fragments in the range of 50-1000 base pairs; exhibits exceptionally high transparency even at high concentrations (4.5%).
• Applications:
• Analytical DNA Gels ≤ 1000bp
• PCR products
• Restriction Enzyme digestion products
• Mutation Analysis
• Blotting Assays ≤ 600bp
• For use with all buffer systems
• Recommended Usage (1X TAE):ConcentrationMolecular Weight1.8%500-1000 bp 3.0%150-600 bp4.5%50-350 bpNote: A 3% agarose gel is equivalent to 8% polyacrylamide
• Source:Gelidium
• Form:White, homogeeous free flowing powder
• EEO:≤0.13
• Clarity (1.5%, NTU):~4
• Absorbance (430nm, 1.5%):≤0.040
• pH (1.5%, H2O, Solution):6-7
• pH (1.5%, H2O, Gel):5.0-7.5
• Ash:≤0.35%
• Sulfate:≤0.1%
• Melting Point (3%):≤78°C
• Gel Point (3%):≤35°C
• Gel Strength (1.5%):≥750g/cm2
• Gel Strength (3%):≥1500g/cm2
• RNase:None Detected
• DNase:None Detected
• Quality Control:Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.
• Functional and Molecular Biology Testing:To Pass Test
• Comparative assay of different size DNA fragments
• Southern Blot (125-23,000bp)
• Background Fluorescence (TAE/TE, EtBr):
• DNA Binding (TAE, EtBr)
• Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase)
• Storage and Stability:Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use.
• Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
• Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.