P38 MAP kinase (MAPK), also called RK (3) or CSBP (2), is the mammalian homologue of the yeast HOG kinase and participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, alpha, beta, gamma and delta, have been identified so far. Like the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3 and SEK activate p38 MAP kinase by phosphorylation at threonine 180 and tyrosine 182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8). Nonphosphorylated p38 MAP Kinase Control Cell.
Western Blots: As controls, we recommend using 15ul of phosphorylated and nonphosphorylated p38 MAP Kinase Control Cell Extracts. Boil for 2 minutes prior to use.
Nonphosphorylated p38 MAP Kinase Control Cell Extracts:Total cell extracts from C-6 glioma cells prepared without anisomycin treatment serve as a negative control.
Phosphorylated p38 MAP Kinase Control Cell Extracts:Total cell extracts from C-6 glioma cells prepared with anisomycin treatment serve as a positive control.
仕様
Size：10Blots
Source Antigen：C-6 glioma cells
Grade：Lysate
Purity：Lysate
Form：Supplied as a liquid in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT and 0.01% w/v bromophenol blue.
