85-1405-80　［取扱停止］Aminopeptidase, Aeromonas, Recombinant (AAP) 100u　A1379-40

特徴

• The Enzyme (29kD) Aeromonas Aminopeptidase is used in the processing of pharmaceutical proteins produces by genetic engineering as well as for physical and structural investigations and for sequence and amino-terminal determinations. This exopeptidase recognizes a specific stop sign at –X- Pro and requires a free a-amino group in the L-configuration. It is therefore suitable for the removal of the redundant N-terminal methionine often added to engineered recombinant proteins.
• Leucine aminopeptidase from Aeromonas proteolytica (AAP) are widely distributed in bacteria, yeast, plant, and animal tissues and, therefore, have a wide variety of biological functions that include protein maturation, protein degradation, hormone level regulation, and cell-cycle control. The importance of understanding the mechanism of action of aminopeptidases is underscored by the recent observation that the naturally occurring peptide analog inhibitor, bestatin, was shown to significantly decrease HIV viral in humans by inhibiting leucine aminopeptidase activity. Another physiologic role for aminopeptidases involves angiogenesis, the formation of new blood vessels, which is essential for tumor growth. Recently, a type-2 methionyl aminopeptidase from eukaryotes was identified as the molecular target for the anti-angiogenesis drugs ovalicin and fumagillin, one of which is in phase III clinical trials. Thus, the inhibition of aminopeptidase activity in viruses and malignant tumors is critically important in preventing the growth and proliferation of these types of cells.
• Activity:~120 Units/mg protein.
• Unit Defenition:One unit is defined as the amount of enzyme that hydrolyses 1umole of L-leucine p-nitroanilide at 25°C per minute.
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.