Factor IXa with the active site irreversibly blocked by the fluorescent inhibitor, Dansyl-EGRck.
Activation of Factor X By Factor IXa: The intrinsic factor Xase complex consists of an enzyme, factor IXa, and a cofactor, factor VIIIa, assembled on a phospholipid surface in the presence of calcium ions. The enzyme complex proteolytically cleaves a MW 10kD activation peptide from the NH2-terminal of the heavy chain of factor X, thus expressing the active-site of factor Xa. Factor IXa is produced from its inactive precursor, factor IX, via proteolytic cleavage by factor XIa or the tissue factor/factor VIIa/phospholipid complex. The activation results from the cleavage of two peptide bonds in the factor IX molecule, releasing an activation glycopeptide with an Mr 10kD. The heavy chain of factor IXa (Mr 28kD) contains the serine protease catalytic domain, while the light chain (Mr 17kD) contains the membrane binding domain.
Factor IXa functions as a serine protease involved in the activation of the zymogen, factor X, to form the enzyme, factor Xa. The factor IXa enzymatic activity is greatly enhanced by inclusion of its cofactor, factor VIIIa, in the presence of calcium ions on a phospholipid surface. Factor IXa is readily inhibited by antithrombin III. This inhibition is greatly accelerated by the presence of heparin. Factor IXa is not inhibited by DFP.
Mode of Action: Enzyme component of the Factor Xase complex
Extinction Coefficient: E1%1cm, 280nm=14.0 (5)
Structure: Two subunits, Mr 28 and 17kD (5), NH2-terminal gla-domain, two EGF domains
Post-translational Modifications: One b-hydroxyaspartate (3), twelve gla residues (4)
Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Products:F0017-20: Factor IXa
F0017-22: Factor IXa with the active site irreversibly blocked by the tripeptide chloromethyl ketone, EGRck.
仕様
Size：100ug
Source Antigen：Human plasma
Grade：Affinity Purified
Purity：≥95%. Factor IXa is prepared from highly purified factor IX by activation with factor XIa, as described by Lindquist et al. (5). The factor IXa is further purified by gel filtration, followed by immuno- affinity purification. Purity is assessed by SDS-PAGE analysis.
