85-1404-65　WNK3, Human (With-No-Lysine Kinase 3) (Control Peptide) 100ug　W2998

特徴

• A 16-aa peptide sequence, mapping near the C-terminus of human WNK-3 .
• The protein kinases represent a superfamily of over 400 members in 57 subfamilies that share a catalytic domain (12 subdomains) of 250-300 amino acids. Some of these residues are conserved and crucial for structure and function of the active site. A conserved lysine, in subdomain II of the catalytic core of all 390 protein kinases, anchors and orientates the a and b phosphoryl groups of ATP. The catalytic domain of WNK (With No K=Lysine) kinases contains all invariant residues, except for this lysine residue. Being specific for multicellular organisms, the number of WNK genes an organism reflects the organism's complexity. There are 4 (identified) WNK genes (WNK1, WNK2, WNK3 and WNK4) in human (h) and rat (r), one in C. elegans and D. melanogaster and no WNK-homologous gene in yeast or bacteria. The hWNK kinases exhibit ~30% sequence identity and 50% sequence homology to human Mitogen Activated Protein kinases (MAPK) STK2, PAK2, MEKK3, PAK3 and Raf-1. The mutation in genes expressing WNK1 (chromosome 12p13.3) and WNK4 (chromosome 17q) causes Pseudohypoaldosteronism type II (PHAII), an autosomal disorder featuring hypertension, hyperkalemia (high serum K+ level), normal/elevated aldosterone level, increased renal salt absorption, and impaired K+ and H+ excretion.
• WNK3 (partial 1450-aa, chromosome Xp11.21-23) is a serine-threonine kinase with a catalytic domain which is ~93% homologous to that of hWNK1. The conserved catalytic lysine in subdomain II is replaced by a cysteine but another lysine residue in the glycine ribbon of the subdomain I confers the WNK3 kinase activity. Eleven out of 33 residues in the subdomains I and II of WNK3 are conserved in the 18 WNK-related kinases. The WNK3 gene is predominantly expressed in brain.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.