A 20-aa peptide sequence within the C-terminus of mouse USF2.
Hepcidin (hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83 aa, rat/human 84 aa) are almost exclusively produced in liver. Human hepcidin is produced from 84-aa precursor, including a putative 24-aa signal peptide. The secreted form of hepcidin in blood and urine is consists of C-terminal 20, 22 or 25-aa residues. The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is upregulated by lipopolysaccharides (LPS).
USF2 (mouse 342 aa, rat 346 aa, human 346 aa, chromosome 19, ~42kD) are ubiquitously expressed nuclear proteins involved in the transcription of a wide variety of cellular genes. USF1, also known as major late transcription factor (MLTF), has significant sequence homology with USF2. USF2 binds to a symmetrical DNA sequence (E-boxes; 5'-CACGTG-3') that is found in a variety of viral and cellular promoters. Effective DNA-binding requires dimerization with another BHLH protein. It binds to DNA as homodimer or heterodimer (USF1/USF2). USF2 is alternatively spliced to two forms: USF2a (rat 346 aa) and USF2b (rat 291 aa.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
仕様
Size：100ug
Source Antigen：Mouse synthetic peptide
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：100% conserved in rat and 94% in human USF2. No significant sequence homology is observed with USF1
