A 14-aa Peptide sequence between the ANK15 and SAM domain of human TANK2 .
The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. Introduction of telomerase into normal human cells has been shown to extend normal cell life by ~ 20 doubling.
Poly(ADP-ribose) polymerases (PARPs) catalyze formation of long, branched chain of poly(ADP-ribose) onto protein acceptors using NAD+ as a substrate. Poly(ADP)ribosylation is a transient posttranslational modification that can either enhance or reduce protein activity. Tankyrase (TRF1 interacting ankyrin-related ADP-ribose polymerase; human 1327 aa, renamed as TNKS-1/TANK1, chromosome 8), a modular protein with homology to ankyrin and poly(adenosine diphosphate-ribose) polymerase (PARP) has been cloned and localized to telomere. The N-terminal HPS domain contains multiple run of histidine, proline, and serine residue homopolymers. TANK1 has 24 ankyrin repeats in TRF-1 interacting domain near the N-terminus. The ANK domain is followed another protein interaction motif called the sterile alpha-module (SAM). The C-terminal region of TANK1 contains the PARP activity. TANK1 uses its ANK domain to bind TRF1 and its PARP domain to ADP-ribosylate itself and TRF1, and thereby inhibiting the ability of TRF1 to bind telomere. The homology between tankyrase and PARPs is limited to catalytic domain. Takyrase-1 is expressed in many tissues and targeted to various intracellular compartments. Tanyrase-1, devoid of NLS (nuclear localization signal), is translocated to telomere (nucleus) through binding of its ANK domain to TRF1. More recently a second tankyrase termed tankyrase-2/TANK2/TNKL, has been cloned and characterized. TANK2 gene (chromosome 10q23.2) encodes a ~130-kD, 1166 aa protein with ~83% identity with TANK1. TANK2 has a unique N-terminus and lacks the HPS domain found in TANk1. TANK2 interacted with TRF1 and displayed APRP activity. It is widely expressed with more abundant expression in skeletal muscle and placenta. Unlike TANK1, TANk2 over expression caused rapid cell death.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Human synthetic peptide
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：The human TANK21 control peptide sequence is not significantly conserved in TANK-1
