Beta-amyloid (Ab) deposition in the brain is the hallmark of Alzheimer's Disease (AD). To initiate Ab formation, beta-secretase cleaves APP at the N-terminus of Ab to release APPsb (~100kD soluble NT-fragment), and C99, a 12-kD CT membrane fragment. Alternatively, alpha-secretase cleaves within the Ab to prevent the formation of Ab. Cleavage by a-secretase produces a soluble N-terminal fragment, APPsa, and a 10-kD membrane C-terminal fragment, C83. Both C99 and C83 can be further cleaved by gamma-secretase releasing Ab and a nonpathogenic p3 peptide, respectively.
Recently TACE, a member of the ADAM family (A Disintegrin And Metalloprotease family) protease has been shown to play a central role in a regulated cleavage of human APP. Inhibition of TACE affects both APP secretion and Ab formation in cultured cells (1). Membrane-bound TNF-a, like APP, is transmembrane protein that can undergo TACE-mediated proteolysis to release the extracellular domain as soluble TNF-a. TACE contain an autoinhibitory domain that must be removed for activity, a proteolytic domain, a disintegrin domain, a cysteine-rich domain, and a Transmembrane domain.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ul
Source Antigen：Recombinant, Rat
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：100% conserved in mouse and 99% in human TACE. No significant sequence homology exists with other proteases
