Control Peptide for S1010-86H (antiserum) and S1010-86K (affinity purified antibody). Synthetic peptide consisting of 16aa sequence from the C-terminal, cytoplasmic domain 6 of human SGLT-1. No significant sequence homology exists with other SGLTs. Species Sequence Homology: Rat, mouse: ~50%.
The kidneys play a major role in the regulation of glucose levels. Kidneys filter ~180 g of glucose per day from the blood. Most of this glucose is reabsorbed back into the blood in the proximal tubules. Typically, glucose is first absorbed within epithelium by a specific transporter protein, Sodium Glucoseco Transporters (SGLT), in the brush-border membrane. It is then transported out of the cell across the basolateral membranes by a facilitated sugar transporter (GLUTs). At least 3 members of SGLTs (SGLT1-3) have been cloned and characterized from various species. Individual member of this family have identical predicted secondary structures with up to 14 transmembrane domains. SGLT1-3 genes code for protein of ~659-672aa residues (calculated size of ~75kD). Both N- and C-termini are predicted to be extracellualr. There is ~60-70% homology between SGLT1-3. SGLTs transport a-methyl-D-glucoside (a-MDG), a non-metabolized model substrate, in Na-dependent manner. SGLT1 does not discriminate a-MDG, glucose and galactose. SGLT2/3 do not transport D-galactose efficiently.
SGLT1/NAGT or SLC5A1/NAGT (rat/mouse 665aa; human 664aa, chromosome 22q13.1, ~75kD) is a high affinity, Na+-coupled, intestinal responsible for active glucose transport across the brush border membrane. In the kidney, SGLT1 is expressed in proximal tubule Sq1 segments. It is also expressed in the intestine. Defects in SGLT1 gene have been implicated in congenital glucose-galactose malabsorption syndrome (GGM). SGLT2/SLC5A2 (rat/mouse 670aa; human 672aa, chromosome 16p11.2) is the low affinity, high capacity Na+-glucose transporter located in the S1 segments of proximal tubules. It is ~60% identical with SGLT1. SGLT2 mediates saturable Na-dependent and phlorizin-sensitive glucose transport. In contrast with SGLT1, SGLT2 does not transport D-galactose. Defect in SGLT2 may be associated with renal glycosuria. SGLT3/SLC54 (pig 660aa; mouse 656/660/616aa; human 659aa, chromosome 22), originally named SAAT1 or pSGLT2, was initially identified in LLC-Pk1 cell line derived from pig renal epithelium. It is also low affinity Na-glucose transporter. It is expressed in kidney, intestine, liver, skeletal muscle and spleen. Like SGLT2, SGLT3 has a low affinity for sugars. It is highly selective for D-glucose and low affinity for D-galactose.
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended Dilution:Antibody Blocking: 5-10ug per 1ul S1010-86H (antiserum) or 1ug S1010-86K (affinity purified antibody)
ELISA: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
