A 20 amino acid peptide sequence within the C-terminus of rat OTR
The hypothalamic Oxytocin (OT) is a nine-amino acid peptide, which exerts multiple biological actions as a hormone and as neurotransmitter. OT stimulates uterine smooth muscle and mammary myoepithelial cell contraction, prostaglandin production by uterine endometrial and amnion cells, milk ejaculation from the mammary gland, and induction of specific mating behavior and maternal behaviors. Just before the onset of labor, uterine myometrium becomes extremely sensitive to oxytocin, for which it is a primary target tissue, because of a dramatic increase in the number of oxytocin receptors. OT initiates its physiological activity by interacting with the G protein-coupled receptor (GPCR) known as oxytocin receptor (OTR). The encoded receptor is a 388-amino-acid polypeptide with 7 transmembrane domains typical of G protein-coupled receptors. Messenger RNAs for the receptor are of two sizes, 3.6 kilobases in breast, and 4.4 kilobases in ovary, uterine endometrium and myometrium. The mRNA level in the myometrium is very high at term.
Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended Dilution:ELISA: 1ug/ml Antibody Blocking: 5-10ug control peptide per 1ug O8251-01 (Affinity Purified Antibody) or 1ul O8251-02 (Antiserum)
Optimal dilutions to be determined by the researcher.
Specificity: The peptide sequence has 95% sequence homology with mouse, 85% with human/monkey, 78% with bovine and 80% with pig OTR. Antibody crossreactivity in various species is not established
Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Rat synthetic peptide
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a liquid in PBS, pH 7.2
Specificity：The peptide sequence has 95% sequence homology with mouse, 85% with human/monkey, 78% with bovine and 80% with porcine OTR. Antibody crossreactivity in various species is not established
