A 14aa Peptide sequence near the cytoplasmic, C-terminus of rat OAT-K1.
Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3), have been cloned and characterized from various tissues. OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus).
A kidney-specific anion transporter termed OAT-K1 (rat 669 aa) has been cloned from rat kidney. OAT-K1 mRNA is specifically expressed in brush-borer membrane of the proximal straight tubules. Rat OAT-K1 (669 aa) shows 72% identity with rat liver oatp. It mediates basolateral uptake of anionic methotrexate but not PAH, taurocholate, and prostaglandin E2. OAT-K2 (498 aa), very similar to OAT-K1 (91% identity), has also been cloned from rat kidney. It also has 62-65% homology with oatp1-3 family of proteins. OAT-K2 is predominantly expressed in convoluted tubules, proximal straight tubules, and cortical collecting ducts. OAT-K2 mediates uptake of hydrophobic anions such as taurocholate, methotrexate, folate, and prostaglandin E2 suggesting that OAT-K2 participate in epithelial transport of hydrophobic anionic compound in the kidney.
仕様
Size：100ug
Source Antigen：Rat synthetic peptide
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Unique to rat OAT-K1. No significant sequence homology is detected with OAT-K2 or other OATs. Available to confirm specificity of antibodies
