Control Peptide for O5401-06 (affinity purified antibody) and O5401-07 (antiserum)
Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic cations from the blood by an active process called "renal secretion". Functional studies have identified two distinct categories of organic cation transporters (OCTs): a system driven by transmembrane potential difference that governs the influx of cations, whereas the H+-gradient-dependent transport system may mediate the efflux of organic cations. Several multispecific, potential-sensitive transporters (OCT1-3) and H+-dependent transporters (OCTN1-3) have been cloned and characterized from various tissues. OCT superfamily of proteins shares high degree of sequence homology, display up to 12 transmembrane domains with cytoplasmic N and C-terminus. Rat OCT2 was initially cloned from kidney by homology screening. OCT2 (rat 593aa, mouse 553aa, human 555aa) shares ~70% homology with OCT1. In rat, it is expressed primarily in kidney, and traces were found in colon stomach, and brain. Rat OCT2 has been localized to the basolateral membrane of S2 and S3 segments of proximal tubules. In contrast, OCT2 was localized to the luminal membranes of distal tubule. OCT2 also mediated uptake of a variety of organic cations.
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended Dilution:Antibody Blocking: 5-10ug per 1ug O5401-06 (affinity purified antibody) or per 1ul O5401-07 (antiserum)
ELISA: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Rat synthetic peptide
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a liquid in PBS, pH 7.5, 0.09% sodium azide.
Specificity：Synthetic peptide consisting of 21aa within the cytoplasmic, c-terminal domain of the rat Organic Cation Transporter 2. No significant sequence homology is detected with other Octs or OctNs. Species Sequence Homology: Mouse: 76%; porcine: 66%; human: 57%.
