Control peptide for O5400-06 (affinity purified antibody) and O5400-07 (antiserum).
Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic cations from the blood by an active process called "renal secretion". Functional studies have identified two distinct categories of organic cation transporters (Octs): a system driven by transmembrane potential difference that governs the influx of cations, whereas the H+-gradient-dependent transport system may mediate the efflux of organic cations. Several multispecific, potential-sensitive transporters (Oct1-3) and H+-dependent transporters (OctN1-3) have been cloned and characterized from various tissues. Oct superfamily of proteins shares high degree of sequence homology, display up to 12 transmembrane domains with cytoplasmic N- and C-terminus. Oct1 (rat/mouse 556aa, ~95% homology; human 554aa, ~78% homology with rat Oct1) is expressed primarily in the kidney, liver, and intestine. Rat Oct1 has been localized to the basolateral membrane of small intestinal enterocytes, hepatocytes, and S1 segment of the proximal renal tubules. Oct1 mediated uptake of TEA was pH and Na+-independent and was reduced when membrane potential decreased. Oct1 also transported NMN, choline, MPP, dopamine, thiamine, noradrenaline, histamine, and spermine but not for putrescine.
Applications:Suitable for use in ELISA and Antidbody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended Dilution:Antibody Blocking: 5-10ug per 1ug O5400-06 (affinity purified antibody) or per 1ul O5400-07 (antiserum)
ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Rat synthetic peptide
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Specificity：Synthetic peptide consisting of 21aa within the cytoplasmic, C-terminus of rat Oct1. The 21aa rat control peptide is 100% conserved in rat Oct1a (430aa), an alternatively spliced form of rat Oct1 (1). No significant sequence homology is detected with other Octs or OctNs.Species Sequence Homology: Mouse Oct/LX-1: 90%; human Oct1: 66%.
