Control peptide for O3002-06 (antiserum) and O3002-07 (affinity purified antibody).
Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N- and C-terminus). Oatp3 (670 aa; 12 TM), cloned from rat retina, shares ~80-83% identity with oatp1-2 and ~77% to rat OAT-K1. Oatp3 is highly expressed in the kidney, and moderately in the retina. Oatp3 mediates uptake of taurocholate, thyroxine, and triiodothyronine. Most of the T4 secreted from the thyroid is deiodinated in peripheral tissues. The liver and kidney are the major sites for the production of T3 and T4. Therefore, oatp3 may play a critical role in transporting thyroid hormones from the circulation to the deiodination sites in the kidney.
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:ELISA: Coat at 1ug/ml
Antibody Blocking: 5-10ug per 1ul O3002-06 (antiserum) or per 1ug O3002-07 (affinity purified antibody)
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Rat synthetic peptide
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Specificity：Synthetic peptide consisting of 12aa sequence within the mid-region of rat Oatp3. No significant sequence homology with other Oatp2 and Oatp3. Species Sequence Homology: Mouse Oatp3: 75%. Human Oatp or LST1: No significant sequence homology.
