Control Peptide for N5377-02G (anitserum) and N5377-02H (affinity purified antibody).
Defensins are a large family of broad-spectrum antimicrobial peptides. There were oringinally identified in leukocytes of rabbits and humans. Defensins are cationic/polar peptides (30-35aa; 3-4kD). They are distinguished by a conserved tri-disulfides and a largely b-sheet structure. Defensins expressed at the cell surface have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules. These granules are released into phagolysosomes where they contribute to the killing of engulfed microorganisms. The genes encoding human alpha- and beta-defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated alpha and beta based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a-defensin (cryptidins), HD 1-6 and two b-defensins, HBD-1 and HBD-2, have been identified. HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides. Alpha-defensins are encoded by genes designated DEFA1-6. Human b-defensins are encoded by the DEFB1 and DEFb2 genes. HD1-4 are expressed in neutrophils. HD5 and HD6 are expressed in epithelial cells of the intestinal and reproductive tract. HD1-3/HNP1-3 make up about 30% of the neutrophil's total granule protein. The HNPs are 29-30aa in length. They are identical in sequence except at the N-terminal amino acid. DEFA1 and DEFA3 differ from each other only by their terminal ala and asp, respectively. DEFA2 is identical to both except that it has 29 instead of 30 amino acids.
Applications:Suitable for use in ELISA, Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended Dilution:ELISA: 1ug/ml per well.
Antibody Blocking: 5-10ug per 1ul N5377-02G (anitserum) or 1ug N5377-02H (affinity purified antibody).
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Human synthetic peptide
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity：Human alpha defensin-1 (HDEFA1) was synthesized from 94aa precursor protein (mature peptide 65-94aa). Synthetic peptide 16aa sequence near the C-terminus of human mature alpha defensin-1. Sequence Homology: Common to human HNP1-3. No sequence homology with human alpha-defensins 4-6 or monkey alpha defensins 1-3.
