85-1403-28　Novel Organic Cation Transporter 1, Mouse, Control Peptide (OctN1) 100ug　N5376-50

特徴

• Control peptide for N5376-51 (affinity purified antibody) and N5376-52 (antiserum). Synthetic peptide corresponding to 20aa within the c-terminus of mouse OctN1. Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic cations from the blood by an active process called "renal secretion". Functional studies have identified two distinct categories of organic cation transporters (Octs): a system driven by transmembrane potential difference that governs the influx of cations, whereas the H+-gradient-dependent transport system may mediate the efflux of organic cations. Several multispecific, potential-sensitive transporters (Oct1-3) and H+-dependent transporters (OctN1-3) have been cloned and characterized from various tissues. Oct superfamily of proteins shares high degree of sequence homology, display up to 12 transmembrane domains with cytoplasmic N and C-terminus. A novel transporter termed OctN1, has been cloned and characterized human fetal liver, which carries a nucleotide birding site motif. OctN1 (human 551aa, mouse 55aa) shares ~32% homology with Oct1-3. It is strongly expressed in adult kidney, trachea, bone marrow, and fetal liver, and several tumor cells, but not in adult human liver. OctN1 mediated uptake of TEA in pH dependent manner.
• Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
• Recommended Dilution:Antibody Blocking: 5-10ug per 1ug N5376-51 (affinity purified antibody) or per 1ul N5376-52 (antiserum)ELISA: 0.5-1ug/ml
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.