An 18 aa peptide sequence from the extracellular, N-terminus region of rat NTR2 (1) was coupled to KLH and antibodies generated in Rabbits.
Neurotensin (NT) is an endogenous tridecapeptide neurotransmitter that influences distinct central and peripheral physiological functions in mammals. Central administration of NT modulates dopaminergic transmission and triggers hypothermic and naloxone-insensitive analgesic responses, whereas peripheral effects include hypotension, decrease gastric acid release, potentiation of lipid digestion. NT is widely distributed throughout the CNS. It has been localized to catecholamine-containing neurons. NT initiates its biological action by interacting with two distinct G-protein coupled receptors (NTR1 and NTR2). Recently, a third receptor NTR3 has been identified that is identical to gp95/sortilin and it is not coupled via the G-proteins. All three receptors bind NT through its C-terminal hexapeptide sequence (8 RRPYIL 13). Biologically active NT (NT8-13) has also been shown to interact with the extracellular domain 3 (between TM6-7) of NTR1.
NTR2/NTRL/NTSR2/NT2 (mouse 417 aa, rat 416 aa; human 410 aa, chromosome 20q1; ~75% interspecies sequence homology) also binds NT. Unlike NTR-1, NTR-2 recognizes, with high affinity, levocabastine, and histamine H1 receptor antagonist previously shown to compete with NT for low-affinity binding sites in brain. NTR2 is ~40 identical with NTR1. It has been suggested that the most prominent biological effects, analgesia and hypothermia, are mediated by NTR2. A non-peptide NT-antagonist SR 48692m which preferentially recognizes the NTR1, did not antagonize these effects. In mouse, it is maximally expressed the cerebellum, hippocampus, and neocortex. Low levels are also found in heart and intestine.
仕様
Size：100ug
Source Antigen：Rat synthetic peptide
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a liquid in PBS, pH 7.2
Specificity：The rat sequence is 100% conserved in mouse and 77% in human NTR2
