Control Peptide for N2177-11 (affinity purified antibody) and N2177-10 (antiserum). No significant sequence homology exist with other neuromedins. Species Homology: Mouse, rat, bovine, canine: 100% G. pig: 92%.
Neurotensin (NT) is an endogenous tridecapeptide neurotransmitter (pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg- pro-Tyr-Ile-Leu-OH) that was originally isolated from bovine hypothalamus. NT influences distinct central and peripheral physiological functions in mammals. Central administration of NT modulates dopaminergic transmission and triggers hypothermic and naloxone-insensitive analgesic responses, whereas peripheral effects include hypotension, decrease gastric acid release, potentiation of lipid digestion. NT causes contraction of smooth muscle. NT is widely distributed throughout the CNS. It has been localized to catecholamine-containing neurons. NT gene (human chromosome 12q21) produces both NT and neuromedin N (NN). In the rat, the NT precursor consists of a 169-aa polypeptide. The signal peptide (1-22 aa) is removed to produce a peptide that is further processed to produce NN (142-147 aa) and NT (150-162 aa). NT sequence is completely conserved in mouse, rat, human, canine, bovine etc. NT initiates its biological action by interacting with two distinct G-protein coupled receptors (NTR1 and NTR2). Recently, a third receptor NTR3 has been identified that is identical to gp95/sortilin and it is not coupled via the G-proteins. All three receptors bind NT through its C-terminal hexapeptide sequence (8 RRPYIL 13). Biologically active NT (NT8-13) has also been shown to interact with the extracellular domain 3 (between TM6-7) of NTR1.
Applications:Suitable for use in ELISA, Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:ELISA: ~1:100,000 using 50-100ng control peptide/well
Antibody Blocking: 5-10ug of Control peptide per 1ug N2177-11 (affinity purified antibody) or per 1ul N2177-10 (antiserum).
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
