Control peptide for N2020-50 (antiserum) and N2020-55 (affinity purified antibody).
Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. At least 7 NHE isoforms (NHE1-7) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The C-terminal domain of NHEs are predicted to be intracellular.
A mammalian homolog of yeast NHA2 has been identified and classified as NHE6 (KIAA0267). NHE6 is 669aa (~75kD), and predicted to contain 12 TM domains within the cytoplasmic N-terminus, the C-terminus may reside in the matrix. NHE6 is ubiquitously expressed but it is most abundant in mitochondrial-rich tissues such as brain, skeletal muscle, and heart.
Applications:Suitable for use in ELISA and antibody blocking experiments. Other applications not tested.
Recommended Dilution:ELISA and antibody blocking experiments: 5-10ug control peptide per 1ug affinity pure IgG or 1ul of antiserum.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Human synthetic peptide
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.
