Control peptide for N2019-03 (antiserum) and N2019-05 (affinity purified antibody).
Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, including hypertension, organ growth and hypertrophy, and regression of cancer and renal intestinal disorders. Five NHE isoforms (NHE1-5) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular.
NHE5 (chromosome 16q22.1) is expressed in human brain, testis, spleen and skeletal muscle. It is involved in pH regulation to eliminate acids generated by active metabolism. It is a major proton extruding system driven by the inward sodium ion chemical gradient (1).
Applications:Suitable for use in ELISA and antibody blocking experiments. Other applications not tested.
Recommended Dilution:ELISA and antibody blocking experiments: 5-10ug control peptide per 1ug affinity pure IgG or 1ul of antiserum.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Synthetic
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a liquid in PBS, pH 7.5, and 0.05% sodium azide.
