A 16-aa peptide sequence within the cytoplasmic, N-terminus of human NBC3.
Bicarbonate, along with CO2, is the major pH buffer of biological fluids. A great majority of HCO3- reabsorption occurs via trans-cellular coupling of the luminal Na-H+-exchanger 3 and Na+-H+-ATPase with the basolateral Na+-HCO3- cotransporters (NBC). Several related proteins constitute the emerging NBC family (NBC1-3) of membrane cotransporters that are found in a variety of epithelial and non-epithelial tissues, and may be tissue specific. Physiologically, NBC is electrogenic, Na+ and HCO3- dependent, Cl- independent, and inhibited by stilbenes (DIDS and SITS). The NBC family of proteins are 30-35% related to anion exchangers (AE2 and AE3; SLC4A1-SLC4A3) and display the same protein topology: (a) At least 10 TM domains with both the N and C-termini predicted to be intracellular, (b) presence of a large, glycosylated, extracellular loop between TM5 and TM6; and (c) the lysine residues are conserved at predicted DIDS-reactive sites.
NBC3 (mouse 1089 aa, human 1044 aa) has 3 three transcripts, which are expressed in a tissue specific manner (9 kb in brain, placenta, lung, liver, muscle, kidney, pancreas, stomach, thyroid, and adrenal etc). NBC3 has two variants. NBC3 is 56% identical with NBC1 and 76% with NBC2.
Applications:Suitable for use in ELISA. Not suitable for Western Blot due to low MW: <3kD. Other applications not tested.
Reccommended Dilutions:ELISA:Coat with 50-100ng Control Peptide/wellAntibody Blocking:Use 5-10ug per 1ul of N0538-15 serum or 1ug of N0538-20 affinity purified
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
