Control Peptide for M9555-01 (serum) and M9555-01 (affinity purified).
Escherichia coli MutY is an adenine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, or A/C mismatches and also has a weak guanine glycosylase activity on G/8-oxoG-containing DNA. The N-terminal domain of MutY, residues 1-226, has been shown to retain catalytic activity. The post-replicative adenine:8-oxo-7,8-dihydroguanine (GO) mismatch is crucial for G:C to T:A transversion. This mismatch is corrected by Escherichia coli MutY which excises the adenine from A:GO. A candidate gene coding for the human counterpart of MutY has been cloned as hMYH (535 aa) (1)
Applications:Suitable for use in ELISA or Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended Dilutions:Antibody Blocking: 5-10ug per 1ul M9555-02 (serum) or per 1ug M9555-03 (affinity purified antibody)
ELISA: Coat plates wtih 50-100ng/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Synthetic peptide
Grade：Highly Purified
Purity：Highly purified.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：A 19-aa synthetic peptide near the C-terminus of human mutY. No significant homology with E. coli mutY (1).
