Human Beta-2 Microglobulin (B2M) is a small (11kD) polypeptide that forms the invariant subunit of the class I HLA-antigens (Human Lymphocyte Antigen) on the cell membrane (1). Increased concentrations of B2M in serum have been found in patients with renal diseases, malignancies (especially of lymphatic origin) and infectious diseases, e.g., AIDS. B2M is the most powerful single prognostic variable in multiple myelome, it is also a good predictor for acute rejection in renal transplantation, furthermore the measurement of B2M is very useful for detecting and monitoring the changes in the glomerular filtration rate in diabetic nephropathy.
Sensitivity:~0.1ug/ml
Assay Time:60 minutes
Kit Components:M5891-01: Microtiter Strip1x96wells*M5891-02: Standard 0ug/ml1x1.5ml*M5891-03: Standard 0.75ug/ml1x1.5ml*M5891-04: Standard 1.5ug/ml1x1.5ml*M5891-05: Standard 3ug/ml 1x1.5ml*M5891-06: Standard 6ug/ml 1x1.5ml*M5891-07: Standard 12ug/ml 1x1.5mlM5891-08: B2M (HRP) 1x15mlM5891-09: Wash Buffer, 50X 1x20mlM5891-10: TMB Substrate 1x15mlM5891-11: Sample Buffer, 5X 1x20mlM5891-12: Stop Solution, H2SO4 1x15mlM5891-13: Low Control 1x1.5mlM5891-13A: High Control 1x1.5ml
Storage and Stability:Store all components except standards at 4°C. Store opened Standards *M5891-02-M5891-07 as aliquots at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
