Control peptide for M2867-75A (antiserum) and M2867-75B (affinity purified antibody).
The cyclic neuropeptide, Melanin-Concentrating Hormone (MCH), plays an important role in food intake and energy balance. MCH stimulates feeding. it is up regulated in obese mice and during fasting. Animals lacking MCH eat less and are leaner. A receptor for MCH (MCHR) has been cloned and characterized. Human MCH receptor is 402aa membrane protein. It is also described as an orphan G-protein coupled receptor SLC-1 or GPR24, somatostatin receptor-like protein. It has 7 transmembrane domains, a Dry motif at the boundary of TM3 and the 2nd intracellular loop, a consensus site for N-linked glycosylation at the N-terminus. MCHR is expressed in ventromedial and dorsomedial nuclei of the hypothalamus. An orphan G-protein coupled receptor (SLC-1, GPR24) has been identified as the receptor of MCH. MCH receptor (MCH1R; human 402aa, rat 353aa) is predicted to contain 7 transmembrane domains, a feature typical of G-protein coupled receptors. Moderate levels of MCHR are also found in eye and skeletal muscle, tongue and pituitary. MCHR binds MCH with sub-nanomolar affinity. It is stimulated by MCH to mobilize intracellular Ca2+ and reduce forskolin-elevated cAMP levels. A second human MCH receptor (MCH2R) has been cloned and characterized. MCH2R gene encodes a 340aa protein with 38% identity with MCH1R. It has the basic characteristics of GPCR and belongs to class 1 (rhodopsin-like) GPCR superfamily. MCH2R transfected cells responds to MCH with an increase in intracellular Ca2+ levels and increased cellular extrusion of protons. MCH2R is mainly expressed in the brain.
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended Dilution:Antibody Blocking: 5-10ug per 1ul M2867-75A (antiserum) or per 1ug M2867-75B (affinity purified antibody)
ELISA: 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Human synthetic peptide
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Synthetic peptide (16aa) near the cytoplasmic C-terminus of human MCHR/GPR24/SLC-1. No significant sequence homology is seen with MCHR2 or with other GPCRs. Species Sequence Homology: Rat, mouse, monkey MCHR1: 100%.
