Control Peptide for N0020-02 (affinity purified antibody) and N0020-01J (antiserum). Synthetic peptide conisting of 17aa sequence located at the predicted cytoplasmic loop near the C-terminus of rat NRAMP2. No significant sequence homology exist with NRAMP1. Species Sequence Homology: mouse NRAMP2 with IRE: 100%; mouse NRAMP2 without IRE: 0%.
Iron is an essential element for a variety physiological process. A variety of proteins are involved in the transport of iron. Most dietary iron exists in the form of ferric iron complexes which must be reduced to yield ferrous ion before it can be taken up by protein transporters such as DCT1 (divalent cation transporter)/NRAMP2/DMT1. These transporters are especially active in small intestine where most dietary iron absorption is conducted. Ferrous Fe (II) is very unstable at physiological pH and quickly oxidized to ferric Fe (III). Highly specialized transmembrane electron transport system, maintained by ferric reductases, is required for the availability of intracellular ferrous ion. A second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568aa, ~65% identity with NRAMP1). NRAMP2 expression is more ubiquitous and has been detected in most tissues. NRAMP2 expression is more pronounced in brain, thymus, proximal intestine, kidney and bone marrow. It is dramatically up regulated by iron starvation in the intestine. NRAMP2 gene produces two alternatively spliced transcripts generated by alternative use of two 3' exons encoding distinct C-termini of the protein as well as distinct 3' untranslated regions (UTRs). One Nramp2 mRNA contains an iron-responsive element (IRE) in its 3'UTR. The IRE is an RNA secondary structure present in the 5'- or the 3'-UTR of animal mRNAs encoding proteins involved in iron metabolism. The second Nramp2 splice isoform (without-IRE, isoform II) encodes a protein in which the C-terminal 18aa of the IRE form (with IRE, isoform I) is replaced by a novel 25aa segment and codes for a distinct 3' UTR lacking the IRE. The two isoforms are differentially localized and regulated in GI tract and kidney. It has been demonstrated that the Nramp2 gene is mutated (Gly185 to Arg at TM4) in both the mk and Belgrade (b) animal models exhibiting a severe microcytic hypochromic anemia marked by a defect in iron absorption by intestinal cells and in erythroid iron use.
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended Dilution:Atnibody Blocking: 5-10ug per 1ug N0020-02 (affinity purified antibody) or per 1ul N0020-01J (antiserum)
ELISA: 1ug/ml to coat ELISA plates
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Rat
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
