85-1401-98　Leptin Receptor Gene Related Protein, Human Control Peptide (OBGRP) 100ug　L1671-24B

特徴

• A 15 amino acid sequence within the first putative intracellular domain of human of human OB-RGRP (1) was coupled to KLH and antibodies generated in rabbits.
• Obesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems. At least five genes, Obese (ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub) have been linked to obesity. Obese gene encodes an adipocyte-tissue derived secreted protein Ob protein/Leptin (167 amino acid, ~16kD) that controls body weight homeostasis. Leptin mediates its effects via the Leptin receptor or Obese receptors (OBR or LR) that is expressed in several tissues including hypothalamus. The Ob-R has at least 6 alternatively spliced forms (OBRa-f or LRa-f) that contain a common extracellular domain. The OBRa represents the initially identified mouse Ob-R (short form, 894aa). OBRa, -c, -d, and -f differ in sequence after Lys889 and have short (30-40 aa) cytoplasmic extension Mouse Ob-Rb (long form) displays ~78% homology to the human Ob-R (long form, 1165aa). OBRb has ~300 aa intracellular tail. Expression of Ob-Rb and other forms have been detected in hypothalamus and other tissues. OBRe lacks the transmembrane domain. The soluble Ob-Re is found in adipose tissues, hypothalamus, heart, and testes. Ob-R is abnormally spliced in db/db mice (truncation of cytoplasmic domain) that are important for leptin signaling.
• More recently, another variant of OBR, termed OB-RGRP (obese receptor gene related protein), has been identified that is distinct from the leptin receptor itself. OB-RGRP transcript contains the first two OBR gene 5'-untranslated exons, but then is alternatively spliced to two either leptin receptor or 131 aa protein OB-RGRP. The OB-RGRP is highly conserved in mouse, rat, human, yeast and C. elegans. OPB-RGRP is highly expressed in the hypothalamic regions that express the OBR mRNA. The OBR/B219, but not the OB-RGRP, is up-regulated in hypothalamus of ob/ob mice. OB-RGRP mRNA is also detected in heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. A new full-length cDNA encoding a new 131 aa protein, leptin receptor overlapping transcript-like 1 gene (LEPROTL1), that is highly homologous with OB-RGRP (~70% identity with OB-RGRP). LEPROTL1 is also widely expressed and has a similar JAK binding site (Pro46-Ile-Pro48) that is conserved in homologous proteins. LEPROTL1 protein is predicted to display 3 TM domains with an extracellualr N-terminus and intracellular C-terminus.