85-1401-54　［受注停止］Hepcidin, 25aa, Human, Control Peptide (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide) 100ug　H2008-52

特徴

• Control peptide for H2008-50 (antiserum) and H2008-51 (affinity purified antibody).
• Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83aa, rat/human 84aa) are almost exclusively produced in liver. Human hepcidin is produced from 84aa precursor, including a putative 24aa signal peptide. The secreted form of hepcidin in blood and urine consists of C-terminal 20, 22 or 25aa residues. The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is upregulated by lipopolysaccharides (LPS).
• Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
• Recommended Dilution:ELISA: 50-100ng control peptide/well
• Antibody Blocking: 5-10ug per 1ug H2008-51 (affinity purified antibody) or per 1ul H2008-50 (antiserum)
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.