Control Peptide for G4000-12 (antiserum) and G4000-12D (affinity purified)
An 18-aa peptide sequence near the amino terminus of mouse/rat GAD67 (1) was synthesized and coupled to KLH.
g-Aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the brain. Glutamic acid decarboxylase (GAD; E.C.4.1.1.15) catalyzes the decarboxylation of glutamate to produce GABA-the rate limiting step in the synthesis of GABA. The two major isoform of GAD, GAD67 (MW 67kD) and GAD65 (MW 65kD) are derived from distinct genes and differ in their subcellular distribution. GAD67 is 594 aa single chain polypeptide encoded by human chromosome 2 gene, whereas GAD65 is am amphiphilic and membrane anchored protein (585 aa, chromosome 10). GAD isoform shares ~65% homology. GAD is also a major autoantigen associated with SMS (stiff-man syndrome).
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Antibody Blocking: 5-10ug per 1ug G4000-12D (affinity purified) or 1ul G4000-12 (antiserum)ELISA: 50-100ng/wellOptimal dilutions to be determined by the researcher..
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
