Peptide sequence is synthetic 22 aa peptide sequence within the cytoplasmic region, C-terminus of mouse GLAR1, KLH conjugated. Peptide sequence has high homology with human (90%) and rat (76%) GALR1. No significant homology is seen with GLAR2, GALR3 or any other G-protein coupled receptors.
Galanin is a 29 aa C-terminally amidated (30 aa, non-amidated in humans), highly conserved but unique neuroendocrine peptide originally isolated from intestine. The first 14 aa are fully conserved in almost all species. Galanin is found in the brain and the gut. It modulates a variety of physiological processed including cognition/memory, sensory/pain processing, neurotransmitter/hormone secretion, and feeding behavior. Several N-terminally elongated (-7-29 and -9-29) or truncated biologically active forms of galanin have also been isolated. Galanin antagonists are chimeric peptides generated by linking the amino terminal portion of galanin to substance P (galantide, M15), bradykinin (M35), the neurokinin antagonist spantide (C7) or an idealized alpha helical region (M40) (see review in refs 2 by Kask et al 1995). Galanin mediated its biological effects by interacting with high affinity cell surface G-protein coupled receptors (GALR1-3). GALR1 (rat 346 aa; human 349 aa; chromosome 15q24; 55-70kD non-glycosylated and glycosylated forms) are 92% conserved between human and rat. GALR1 is expressed in small intestine, heart prostate, and several areas of the brain (ventral hippocampus, amygdala, supraoptic nucleus, hypothalamus, etc). GALR1 has high affinity for galanin (Kd=0.07 nM), N-terminal galanin fragments, and putative galanin receptor antagonists galantide, C7, M35, and M40. C-terminal galanin fragments do not bind to GALR1.
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:ELISA: 50-100ng/ wellAntibody Blocking: 5-10ug control peptide per 1ug affinity purified antibody (G1043-05D) or 1ul serum antibody (G1043-05H).
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
