Fatty acids are important for general cellular metabolism. A number of proteins have been implicated in the transport and storage of fatty acid. FABPs (fatty acid binding proteins) are a group of cytoplasmic, small mol wt (14-15kD). FABP’s have a widespread tissue distribution. FABPs are quite abundant (3-5% of total cellular protein). At least 7 FABPs, FABP1-7, have been cloned and characterized from various tissues. FABPs can bind long-chain fatty acid, fatty-acid acyl-CoA and acyl-L-carnitine. Several different isoform of FABP have been identified. These isoforms are generally referred to by tissue type (liver, heart, intestine, adipocyte, kidney, brain etc. For example, protein designated as H-FABP indicates that it is heart type). Expression of these isoform is not exclusive. More than one isoform can be found in a given cell or tissue. Three main types of FABPs that were initially discovered in the heart (FABP-H), liver (FABP-L), and intestine (FABP-I) are not exclusive these tissues and show considerable differences at the amino acid level (~30% identity). Other FABPs detected in adipocyte, kidney, and brain show a high degree of sequence homology among each other and with other FABPs. FABPs are also known as mammary derived growth inhibitor (MDGI), adipocyte lipid binding protein (ALBP), Myelin protein P2 homolog, P2 adipocyte protein, 422 protein (P15). Human FABP-H or adipocyte is 132aa protein (chromosome 2p11). Rat and mouse adipocyte-FABPs are 133aa single polypeptide chains.
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended Dilution:ELISA: 50-100ng per well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Human synthetic peptide
Grade：Highly Purified
Purity：Highly purified (≥ 98%).
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Synthetic peptide corresponding to aa70-81 (EFDEVTADDRKVS) of the C-terminal human FABP. Isoform Sequence Homology: Human adipocyte lipid binding protein (hALBP, 132aa), mouse/rat H-FABPs: 100%; human muscle FABP, porcine and bovine H-FABPs: 91%. No significant homology is seen with other isoforms of FABPs. The sequence is also quite conserved in species as diverse as fish and C. elegans FABP.
