Control peptide for D9020-18 (antiserum) and D9020-19 (affinity purified antibody). Synthetic peptide consisting of 20aa sequence within the C-terminus of human DAT. This region is presumably from the cytoplasmic domain. No significant sequence homology is seen with other transporters. Species Sequence Homology: Monkey: 100%; mouse: 90%, 19/20aa; rat: 80%, 16/20aa DAT.
Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4). The two isoforms of D2R, D2 long (D2L) and short (D2S), are encoded by splice variants of a single gene and differ only by the presence of an additional 29 AA in the intracellular domain 3 of the D2 long form. It may play a role in the coupling of the receptor to G-proteins. All members of this family have similar structure and contain 7 putative transmembrane domains. A given cell or tissue may express more than one DR. Specific radioligands do not exist that can differentiate between these DR. Therefore, specific antibodies are needed to distinguish, localize, and document changes in DR levels in cells and tissues under various normal and pathological conditions. Dopamine transporter (DAT) is plasma membrane transporter for the termination of dopaminergic neurotransmission by its high affinity Na+ and Cl-reuptake of dopamine into presynaptic terminals. This protein is the target of psychomotor stimulants such as amphetamines or cocaine. DAT (human 620aa; rat/mouse 619aa glycoprotein) is predicted to contain 12 transmembrane domains with N- and C-termini in the cytoplasm.
Applications:Suitable for use in ELISA and Antibody Blodkiing. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended Dilution:Antibody Blocking: 5-10ug per 1ul D9020-18 (antiserum) or per 1ug D9020-19 (affinity purified antibody)
ELISA: 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Human synthetic peptide
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
