Rat D1A receptor is a 446 aa, G-protein coupled, and transmembrane receptor protein (1). A 13aa Peptide (Control peptide) sequence from the rat D1A receptor within the C-terminal cytoplasmic domain. The 13 aa D1R peptide showoed no significant sequence homology with other dopamine receptors (D1B, D2-D5). Species sequence homology: 100% with porcine/ bovine, 77% with human.
Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4). The two isoforms of D2R, D2 long (D2L) and short D2S), are encoded by splice variants of a single gene and differ only by the presence of an additional 29aa in the intracellular domain 3 of the D2 long form. It may play a role in the coupling of the receptor to G-proteins. All members of this family have similar structure and contain 7 putative transmembrane domains. A given cell or tissue may express more than one DR. Specific radioligands do not exist that can differentiate between these DR. Therefore, specific antibodies are needed to distinguish, localize, and document changes in DR levels in cells and tissues under various normal and pathological conditions.
Applications:Suitable for use in ELISA, Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:ELISA: Coat with 1ug control peptide/well
Antibody Blocking: Use 5-10ug control peptide per 1ug of D9011-06 (affinity purified antibody) or 1ul D9011-05 (antiserum).
Optimal dilutions to be determined by the researcher.
仕様
Size：100ug
Source Antigen：Rat synthetic peptide
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
