A 21 aa peptide sequence within the C-terminus of mouse CRY1. 85% conserved in monkey, human, 76% in chicken and 71% in frog CRY1. No significant homology is seen with CRY2. Control peptide, because of its low MW (<3kD), is not suitable for Western Blot.
Circadian rhythm is one of the most fascinating and complex biological phenomenon's. The circadian clock controls biological activities on daily light-dark cycles in species from cyanobacteria to humans. The circadian clock has three major components: A photoactive pigment (chromophore) for sensing light and transmitting light signals, the circadian clock that oscillates every ~24-hrs, and the genes controlled by the circadian clock to bring about the physiological and behavioral changes. Several genes (Drosophila Clock Per, Tim; mammalian Per1, Per2, Per3, MOP3 and MOP4, and BMAL1) have been linked to rhythmicity or circadian behavior of living organisms. However, very little is known about the mammalian photosensory molecules.
Most recently mammalian homolog of the plant blue-light photoreceptors termed cryptochromes have been identified. Mouse CRY1 and CRY2, are 606 aa and 569 aa protein, respectively. Cry1 and Cry are specifically expressed in ganglion cell and inner nuclear layers of the mouse retina. CRY1 is expressed at high level in the SCN and oscillates in a circadian manner. Like other genes implicated in circadian mechanism, CRY1 and CRY2 are also expressed in most animal tissues. Therefore, mammalian cryptochromes appears to play an important role in entrainment of the circadian clock.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blotting, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. This antibody has been cited in refs. (2)
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Immunohistochemistry & Immunofluorescence: We recommend the use of aff purified IgG at 2-10ug/ml in 4% PF-fixed and in frozen free floating sections (See published reports on this antibody in refs. 3)
Immunoprecipitation: This antibody has been used for IP (see Lee C et al in refs 2).
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
