Pentraxins family of proteins acquired the name from their ability to form pentameric (or decameric) structures formed by non-covalent interactions. C-reactive protein (CRP or PTX1; mature chain 206aa; chromosome 1q21-23) nonglycosylated, ~24kD monomer and ~118kD pentamer) is a ubiquitous protein found in both vertebrates and invertebrates. Originally CRP was defined as a substance, observed in the plasma of patients with acute infections, that reacted with the C polysaccharide of the pneumococcus. It is one of the plasma proteins that are called acute phase reactants because of a pronounced rise in concentration after tissue injury or inflammation; in the case of CRP the rise may be 1000-fold or more. CRP is composed of 5 identical, 21,500D molecular weight subunits. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver; those cells produce CRP detectable on lymphocytes.
Applications:Suitable for use in Western Blot and ELISA. Other applications have not been tested.
Recommended Dilutions:Western Blot: 50-200ng/laneELISA: 1ug/ml (Coat or as standard)
Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
仕様
Size：100ug
Source Antigen：Human serum
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography (≥ 95% by RID, SDS-PAGE, IEP).
Form：Supplied as a liquid in 0.1M Tris, 0.2M sodium chloride, 2mM CaCl2, pH 7.5, 0.09% sodium azide.
