85-1399-86　CNG1, Rat (Cyclic Nucleotide-Gated Channels 1) Control Peptide 100ug　C5999-10

特徴

• Control Peptide for C5999 (antiserum) and C5999-05 (affinity purified antibody).
• The cyclic nucleotides cAMP and cGMP are implicated in signal transduction events such as the visual transduction, relaxation of smooth muscles, intestinal secretion of water and salt, reabsorption of Na+ and water in the distal tubule of the nephrons. cAMP/cGMP activate Ca2+ -permeable ion channels called cyclic nucleotide-gated channels (CNG or CNC). Activation of CNG leads to depolarization of the membrane voltage and to a concomitant increase of the cytosolic Ca2+. CNG consists of two distinct subunits, designated alpha and beta subunits. Several CNG a-subunits (CNG alpha 1-3) and b-subunits (subunit 2 or CNGb1-2 or CNG4-5) and numerous isoforms. a-subunit can form functional channel by themselves. b-subunits modulate the channel property of a-subunits. CNG display intracellular N and C-termini, 6 transmembrane domains or segments (S1-S6). The region between S5 and S6 contains the ion-conducting pore (P). The cyclic nucleotide-binding region is found at the c-terminus. Native functional CNG may exists as heteromultimer containing some combination of a and b subunits.
• Bovine CNG1 was initially cloned and characterized from rod cells. CNG1 (rat 683aa, mouse 684aa, human 686aa; also describes as OCNC1) is primarily expressed in outer segment of photoreceptor rod cells, and in a variety of tissues (kidney, eye, pineal gland, pituitary, adrenal, spleen, brain, heart, skeletal muscle and testis). Human/mouse CNG1 exhibit ~88% sequence homology
• Applications:Suitable for use in ELISA, Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.
• Recommended Dilution:ELISA: 0.5-1ug/mlAntibody Blocking: 5-10ug per 1ul C5999 (antiserum) or 1ug C5999-05 (affinity purified antibody)
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.