Control peptide for C5837-50 (antiserum) and C5837-52 (affinity purified antibody).
Chloride is a critical component of all living cells. Voltage-gated chloride channels regulate cellular traffic of chloride ion. The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport. Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsen's disease), cystic fibrosis, Bartters syndrome type III, Dent's disease and X-linked recessive nephrolithiasis. In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb). Additional forms of these proteins are obtained by alternative splicing. All CLC proteins (~700-1000aa) are predicted to contain 10 (possibly 12) transmembrane domains. CLC-1 and CLC-K1/K2 that are specific for kidney. Most other CLC are widely distributed in various tissues. Rat CLC-7 is 802aa membrane protein (mouse 803aa, human 805aa) (1). CLC-7 has a calculated MW of ~90kD. CLC-7 is ~45% identical with CLC-6. CLC-7 is expressed in brain, testes, muscle and kidney (1).
Applications:Suitable for use in ELISA, Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended Dilution:Antibody Blocking: 5-10ug per 1ul C5837-50 (antiserum) or 1ug C5837-52 (affinity purified antibody)
ELISA: 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Rat synthetic peptide
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Synthetic peptide 23aa sequence near the C-terminus of rat CLC-7. No significant sequence homology is detected with other CLCs or other proteins. Species Crossreactivity: Rat. Species Sequence Homology: Mouse: 100%; human: 95%.
