Control peptide for A3000-14 (affinity purified antibody) and A3000-17A (antiserum).
Rat AQP4 is a 301 amino acid transmembrane protein. It belongs to the MIP family. Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin”. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. Human Aquaporin-0 is a 263 amino acid transmembrane protein belonging to the MIP family. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N- and C-terminus are predicted to be cytoplasmic.
Applications:Suitable for use in ELISA, Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended Dilutions:Antibody Blocking: 5-10ug per 1ug A3000-14 (affinity purified antibody) or per 1ul A3000-17A (antiserum)
ELISA: 1ug/mlOptimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Rat synthetic peptide
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Synthetic peptide 17aa sequence corresponding to aa280-296 in the region near the carboxy terminus of the rat AQP4 (1,2). This peptide is predicted to be cytoplasmic (2). peptide sequence is unique to AQP4. Species Sequence Homology: Mouse: 94%; Human: 82%.
