A 15aa Peptide sequence near the cytoplasmic C-terminus of human AE4.
Control Peptide for A2295-14G (antiserum) and A2295-14G1 (affinity purified)
Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300 aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself.
Recently, a new member of AE family, AE4, has been clones from rabbit and human. Human AE4 (945 aa; chromosome 5) is predicted to have 14 TM domains. It is strongly expressed in the kidney. Rabbit and human AE4 share 84% identity.
Recognizes human AE4. Sequence homology: Human isoforms 1AE, 2, 3, NBC5 (AB032762, 657 aa) and NBC (AF313465, 990aa; XM_038736, 959aa)-100%. Species sequence homology: rabbit-100%
Applications:Suitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended Dilution:Western Blotting: 1:1000-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer..
