Control peptide for A2295-07W (affinity purifed antibody) and A2295-07V (antiserum). Synthetic peptide consisting of 18aa sequence mapping near the C-terminus of human AT2. This peptide is predicted to be cytoplasmic.
Angiotensin II mediates its action by interacting with membrane receptor, Angiotensin II type 1 receptors. The signal is transmitted via G-proteins that activates a phosphatidylinositol-calcium second messenger system. ATII receptors are integral membrane proteins of approx. 359-363aa and predicted to contain at least 7 transmembrane domains. The N and C-terminus are predicted to extracellular and cytoplasmic, respectively. Three isoforms of ATII receptors have been cloned. In rat type 1a (359aa) is expressed in the liver kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscles (1). Rat Type 1b (also known as AT3; 359aa) is found in liver, kidney, and aorta (2). Type 1c (177aa) has been detected in brain, kidney, liver, spleen and lung (3).
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended Dilution:Antibody Blocking: 5-10ug per 1ug A2295-07W (affinity purifed antibody) or 1ul A2295-07V (antiserum)
ELISA: 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Human, synthetic peptide
Grade：Highly Purified
Purity：Highly purified
Form：Supplied as a liquid in PBS, pH 7.5, 0.09% sodium azide.
Specificity：No sequence homology with other G-protein coupled receptors.Species Sequence Homology: Rabbit: 100%; Rat: 88% (16/18aa); Mouse: 83% (15/18aa) AT2 receptors.
