100% conserved in mouse and human. No significant homology is seen with AKT2 or AKT1 or other kinases
AKT3 or Protein Kinase gamma (RAC-PK-g) is highly related to other members of RAC protein kinase family. It is abundantly expressed in testes and brain and is involved in regulation of cellular growth. Rat AKT3 is 454 aa (human 479 aa, mouse 479 aa). An 11 aa peptide sequence (designated AKT31-P, control peptide) near the N-terminal regulatory domain of rat AKT3.
Putative human homologs of the protooncogene v-akt of the acutely transforming retrovirus AKT8 have been cloned. These protein-serine/threonine kinase proteins have a catalytic domain closely related to both PKA and PKC and have been designated rac (related to A and C kinases), pkb (Protein kinase B) or Akt. RAC protein kinase family members feature pleckstrin homology (PH) domain at the amino terminus and a protein-serine/threonine kinase catalytic domain at the carboxy terminus. The Amino terminal domain (referred to as AH-Akt Homology domain) spans from 1-148 amino acids and contains the PH domain, a region found in diverse group of signaling proteins. The PH domain (amino acids 1-106) has been implicated in interactions with other proteins such as G-protein bg subunits, as well as phosphoinositides. The kinase domain is located between residues 148 to 411. These enzymes are activated by diverse ligands such as PGDF, EGF and basic FG in NIH 3T3, Rat-1 or Swiss-3T3 cells.
