Control Peptide for N2801-01 (antiserum) and N2801 (affinity purified)
A 15 amino acid peptide sequence near the C-terminus of rat NKCC2 (1)
Corresponding antibody N2801 recognizes rat Sodium, Potassium, Chloride Cotransporter 2 (NKCC2, BSC1, CCC2). The immunogen shows no significant sequence homology with NKCC1 or TSC. Species Crossreactivity: Rat. The immunogen sequence is 100% conserved in mouse, 87% in human and rabbit.
Chloride is a critical component of all living cells. It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions. The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane. It includes the thiazide-sensitive Na+Cl- cotransporters (NCC or TSC), the loop diuretics-sensitive Na+K+Cl- (NKCC) cotransporters (NKCC1/CCC1/BSC2 and NKCC2/CCC2/BSC1), and the K+Cl- cotransporters (KCC1-4). These co-transporters share a common predicted membrane topology, with 12 TM domains (~500 aa), and long hydrophilic, intracellular N-and C-termini containing regulatory phosphorylation sites. NKCC transport Na, K, and Cl ions into and out of a wide variety of epithelial and non-epithelial cells. The transport process is characterized by electroneutrality (almost always with stoichiometry of 1Na:1K:2Cl) and inhibition by the loop diuretic bumetanide, benzametanide, and furosemide.
Unlike NKCC1, NKCC2 (human 1099 aa, rat/mouse 1095 aa; calculated mol size of ~121kD; actual size ~150kD; ~45% identity with NKCC1) is most strongly expressed in the kidney (inner and outer stripes of medulla and cortical thick ascending limb), and macula densa. NKCC2 plays a critical role in transcellular absorption of Na+Cl- by the medullary and cortical TALH, and a secondary role in the paracellular transport of Na-Ca and Mg. Mutations in the NKCC2 gene result in Bartter's syndrome, an inherited disease characterized by hypokalemic metabolic alkalosis, hypercalciuria, salt wasting, and volume depletion.
Applications: Suitable for use in ELISA and Antibody Blocking. Other applications not tested.
Recommended Dilution:Elisa: Coat plates at 1ug/mlAntibody Blocking: 5-10ug per 1ul N2801-01 (antiserum) or 1ug N2801 (affinity purified).
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
