Protein Western Blots are manufactured by using high-quality protein isolated from documented tissues. The tissue is excised, immediately frozen, and then pathologically identified. Protein is isolated by proprietary techniques. Each lane of the blot contains 50ug of protein.
The protein is separated by 4-20% gradient gel electrophoresis and transferred to a PVDF membrane. Human placenta protein is used as an interblot control lane for protein expression comparison between different blots.
Protein Western Blots are supplied ready for immediate use, so that you can immediately screen for your protein of interest without the hassle of obtaining hard to find tissues and processing the protein yourself.
Features:Ready to use, pre-blotted with high quality proteinSuitable for both radioactive and non-radioactive probesExtensive selection of blotsDocumentation of tissues' clinical histories is available (additional cost)“Matched" tissue slides or cDNA might be available for immunohistochemistry, in situ hybridization or PCRHigh sensitivity and low background Normalized based on amount of protein used in blots Interblot control built into every blot
Applications:Identification of normal, tumor or disease tissues specific gene expressionProtein size determinationGene expression pattern analysisCompare expression level of novel genesExamining alternative splicing and prematur etermination of specific proteins
Tissues on Blots (different tissues, different single donors)lane 1: heartlane 2: brainlane 3: kidneylane 4: liverlane 5: lunglane 6: pancreaslane 7: spleenlane 8: skeletal musclelane 9: stomachlane 10: small intestinelane 11: colonlane 12: rectumlane 13: uteruslane 14: prostatelane 15: testislane 16: placenta
Storage Conditions:Store blots at 4°C. Store moist in a sealed bag. Keep away from light.
Note: USBio recommends following protocol provided. Has not been evaluated for compatibility with other blocking and stripping methodologies or reagents.
