Tissue Protein Lysate produced under consistent processing conditions to ensure intact structure and original nature. Total protein is prepared from whole-tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
Applications:Suitable for use in Electrophoresis, Western Blot, Immunoprecipitation, Enzymatic Activity, Protein-DNA Interaction, Protein-Protein Interaction, Tissue-Specific Expression Identification. Other applications not tested.
Recommended Dilutions:Western Blot: 10-20ug/lane Optimal dilutions to be determined by the researcher.
Quality Control:1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot
2. The isolated protein is Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Human
Grade：Molecular Biology Grade
Form：Supplied as a liquid in HEPES, pH 7.9, KCl, sucrose, sodium deoxycholate, NP-40.
