Tissue Protein Lysate produced under consistent processing conditions to ensure intact structure and original nature. Total protein is prepared from whole-tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
Applications:Suitable for use in Electrophoresis, Western Blot, Immunoprecipitation, Enzymatic Activity, Protein-DNA Interaction, Protein-Protein Interaction and Tissue-Specific Expression Identification. Other applications not tested.
Recommended Dilution:Western Blot: 10-20ug/laneOptimal dilutions to be determined by researcher.
Quality Control:1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.
Storage and Stability:Store the protein lysate at -70°C for long term storage. Stable for 6 months after receipt. After the protein is thawed, store it at 4°C for continuous use. Avoid freeze and thaw cycles.
Additional Data Supplied: All tissue protein samples are supplied with verified donor age and sex.
仕様
Size：100ug
Source Antigen：Human
Grade：Molecular Biology Grade
Form：Supplied in a buffer including Tris-HCl, sodium chloride, Triton X-100, glycerol and a cocktail of protease inhibitors.
