Tissue Protein Lysate produced under consistent processing conditions to ensure intact structure and original nature. Total protein is prepared from whole-tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
Quality Control:1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot
2. The isolated protein is Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.
Applications:Suitable for use in Electrophoresis, Western Blot, Immunoprecipitation, Enzymatic Activity, Protein-DNA Interaction, Protein-Protein Interactionand Tissue-Specific Expression Identification. Other applications not tested.
Storage and Stability:For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months
仕様
Size:1mg
Source Antigen:Human
Grade:Molecular Biology Grade
Form:Supplied as a frozen liquid in HEPES, pH7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium deoxycholate, NP-40 and a cocktail of protease inhibitors.