Tissue Protein Lysate produced under consistent processing conditions to ensure intact structure and original nature. Total protein is prepared from whole-tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
Applications:
Electrophoresis, Western Blot and Immunoprecipitation
Enzymatic Activity
Protein-DNA Interaction
Protein-Protein Interaction
Tissue-Specific Expression Identification
Quality Control:1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot
2. The isolated protein is Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Source Antigen：Human
Grade：Molecular Biology Grade
Form：Supplied as a frozen liquid in HEPES, pH7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium deoxycholate, NP-40 and a cocktail of protease inhibitors.
