Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC.
Applications:Suitable for use in Electrophoresis, Western Blot, Immunoprecipitation, Enzymatic Activity, Protein-DNA Interaction, Protein-Protein Interactionand Tissue-Specific Expression Identification. Other applications not tested.
Quality Control:1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot
2. The isolated protein is Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.
Storage and Stability:For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. Further dilutions can be made in assay buffer.
Additional Data Supplied: All tissue protein samples are supplied with verified donor age and sex.
仕様
Size:1mg
Source Antigen:Human
Grade:Molecular Biology Grade
Form:Supplied as a frozen liquid in HEPES, pH7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium deoxycholate, NP-40 and a cocktail of protease inhibitors.