BioGenomics™ Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.
The Matched Pair contains 2 x 10ul of PCR Ready First Strand cDNAs from normal and diseased tissues.These cDNAs are ideal for analyzing gene expression patterns and discovering novel genes. All cDNAs are made under optimal and consistent conditions, which ensure that representation is maintained from total RNA to cDNA.
Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 11ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul final volume. RT reaction was stopped by heating at 65°C for 10 minutes. The cDNA is in 1X RT buffer (1X RT Buffer: 50 mM Tris Cl, pH 8.3, 75mM KCl, 3mM MgCl2, 10mM DTT).
T5595-089 consists of cDNAs isolated from primary tumor (or diseased tissue) and its adjacent normal tissue. cDNAs in the pair are prepared from the same donor. The estimated cDNA concentration is about 2.5 ng/ul. 1ul cDNA is sufficient for one PCR reaction.
Features:Ready to use for PCR quantification of gene expression Oligo dT primer used to ensure the entire 3' end of cDNA is present With some cDNA used as templates, 12kb PCR amplicon was obtained to ensure intact cDNAs The largest selection of cDNAs from different tissues on the market Placenta cDNA is included in every panel as an interpanel control Documentation of tissues' clinical histories available (additional cost) Economical way to get cDNA from multiple tissues
Applications:Immediate PCR amplification of known genes for their expression Quantification of low copy gene expression in multiple tissues Identification of tissue-specific expression of target genes Analysis of gene expression in rare tissues Verification of genetic mutation Comparison of a specific gene between different tissues Analysis of mRNA alternative splicing
