Control peptide for Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS) cat#s: N5350-28, N5350-28B, N5350-29.
15 amino acid peptide sequence corresponding to human eNOS. Human, rat, mouse and bovine eNOS/NOS-3 are ~1202-1205aa proteins
Corresponding antibody recognizes the 135-140kD protein endothelial nitric oxide synthase (eNOS, NOS III). Sequence is 100% conserved in mouse/bovine (15/15 aa), 93% in rat (14/15 aa) and 86% in pig (13/15 aa). No significant sequence homology is seen with NOS-1/NOS-2 or other proteins.
Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues.
NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-III, mol wt. ~130-160kD). One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain and platelets. The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II). Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible. Sequence homology among different cloned isoforms is ~ 50%.
Applications:Suitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended Dilution:ELISA: Control peptide should be coated at 1ug/ml
Western Blotting: 1:100-1:1000. Recognizes 135-140kD protein
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
